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2 edition of Carbonic anhydrase in arabidopsis thaliana: role and regulation of expression. found in the catalog.

Carbonic anhydrase in arabidopsis thaliana: role and regulation of expression.

Fernando Ferreira

Carbonic anhydrase in arabidopsis thaliana: role and regulation of expression.

by Fernando Ferreira

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  • 17 Currently reading

Published .
Written in English


The Physical Object
Pagination1 v.
ID Numbers
Open LibraryOL19071855M
ISBN 100612690970

Carbonic anhydrase activity. The activity of carbonic anhydrase was determined by a modified Wilbur-Anderson assay [28]. Leaf tissue ( µg of fresh weight) was ground in liquid nitrogen and homogenized with 2 ml of 50 mM HEPES buffer (pH ), containing 10 mM DTT, mM EDTA and % Triton X (v/v) and 10% glycerol (v/v).Author: Jolanta Floryszak-Wieczorek, Magdalena Arasimowicz-Jelonek. Characterization and Expression of Two cDNAs Encoding Carbonic Anhydrase in Arabidopsis thaliana' lanette P. Fett and John R. Coleman* Department of Botany, University of Toronto, 25 Willcocks Street, Toronto, Ontario, Canada M5S 3B2 Two distinct cDNA clones encoding carbonic anhydrase (CA) were isolated from an Arabidopsis thaliana XYES.

Summary Carbonic anhydrase (CA) is an abundant protein in most photosynthesizing organisms and higher plants. This review paper considers the physiological importance of the more abundant CA isofor Cited by: 5. was used to measure the activity of carbonic anhydrase using the method with modifications [28]. CA activity was measured at °C in a container consisting of ml M baritone buffer (5, 5-diethylbarbituric acid; pH ), ml sample, and 3 ml CO 2-saturated H 2 O. The carbonic anhydrase activity was defined as WA = (t 0 /t) -1 Author: Hongtao Hang, Yanyou Wu.

  Dark respiration causes an increase in leaf CO2 concentration (Ci), and the continuing increases in atmospheric [CO2] further increases Ci. Elevated leaf CO2 concentration causes stomatal pores to close. Here, we demonstrate that high intracellular CO2/HCO3− enhances currents mediated by the Arabidopsis thaliana guard cell S-type anion channel SLAC1 upon coexpression of any one of Cited by: So far, it is known that in the immune response of plants to pathogens carbonic anhydrase plays a very important role, constituting a salicylic acid (SA) binding protein 3-SABP3 [11]. Interestingly, the used acetazolamide (a known CA inhibitor) inhibited the in vitro CA carbonate activity of Author: Jolanta Floryszak-Wieczorek, Magdalena Arasimowicz-Jelonek.


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Carbonic anhydrase in arabidopsis thaliana: role and regulation of expression by Fernando Ferreira Download PDF EPUB FB2

Carbonic anhydrase (CA, EC ), catalyzes the interconversion of CO 2 and HCO3’ in many organisms. The majority of CA activity in C3 leaves is in the chloroplast and expression of the chloroplast CA is regulated by light.

To investigate the physiological role of the chloroplast CA, its expression Cited by: 3. Fett JP and Coleman JR () Characterization and expression of two cDNAs encoding carbonic anhydrase in Arabidopsis thaliana. Plant Physiol – CrossRef PubMed Google Scholar Forsman C and Pilon M () Chloroplast import and sequential maturation of pea carbonic anhydrase: The roles of various parts of the transit by:   A possible role for cytoplasmic CA in the plant cell is discussed.

Two distinct cDNA clones encoding carbonic anhydrase (CA) were isolated from an Arabidopsis thaliana [lambda]YES library.

One of these clones, CA1, encodes a kD polypeptide and is essentially the same as a previously reported Arabidopsis CA cDNA (C.A. Raines, P.R.

Horsnell, C. Holder, J.C. Lloyd [] Cited by: The Role of Carbonic Anhydrase in Photosynthesis. Multiple Roles of Carbonic Anhydrase in Cellular Transport and Metabolism Biocontrol of Bacillus subtilis () on Arabidopsis thaliana roots by forming protective biofilms against gram-negative bacteria Pseud Figure 2: Rhizospheric chemical warfare: schematic representation of Cited by:   Here we show that recently isolated Arabidopsis thaliana β-carbonic anhydrase double mutants (ca1 ca4) 6 exhibit an inversion in their response to elevated CO 2, showing increased stomatal Cited by:   Red alga, Gracilariopsis chorda, contains seven carbonic anhydrases that can be grouped into α- β- and γ-classes.

Carbonic anhydrases (CAHs) are metalloenzymes that catalyze the reversible hydration of CO2. These enzymes are present in all living organisms and play roles in various cellular processes, including photosynthesis. In this study, we identified seven CAH genes (GcCAHs) Author: Md.

Abdur Razzak, JunMo Lee, Dong Wook Lee, Jeong Hee Kim, Hwan Su Yoon, Inhwan Hwang. Encodes a putative beta-carbonic anhydrase betaCA1.

Together with betaCA4 (At1g) regulates CO2-controlled stomatal movements in guard cells, as well as attenuates immunity. Differential CA gene expression in response to changing atmospheric CO2 conditions contribute to altered disease resistance levels. Carbonic Anhydrase Carbonic anhydrases are a widespread class of enzymes found in all domains of life that catalyze the physiologically important reactions of carbon dioxide hydration and the reverse reaction, bicarbonate dehydration, providing rapid approach to equilibrium between dissolved CO2 and bicarbonate ion in aqueous solutions The role of α-carbonic anhydrase 4 (α-CA4) in photosynthetic machinery functioning in thylakoid membranes was studied, using Arabidopsis thaliana wild type plants (WT) and the plants with.

Excitation energy transfer and charge separation are affected in Arabidopsis thaliana mutants lacking light-harvesting chlorophyll a/b binding protein Lhcb3 Source JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY.

Increased Expression of Mitochondria-localized Carbonic Anhydrase Arabidopsis thaliana Chunyun Jiang Both βCA1 and βCA4 play a role in the regulation of. We demonstrate that Arabidopsis thaliana double-mutant plants in the β-carbonic anhydrases βCA1 and βCA4 show impaired CO2-regulation of stomatal movements and increased stomatal density, but Cited by: Importance of Post-Translational Modifications for Functionality of a Chloroplast-Localized Carbonic Anhydrase (CAH1) in Arabidopsis thaliana Stefan Bure´n1¤, Cristina Ortega-Villasante2, Amaya Blanco-Rivero2, Andrea Martı´nez-Bernardini1,2, Tatiana Shutova1, Dmitriy Shevela3, Johannes Messinger1,3, Laszlo Bako1, Arsenio Villarejo2,Go¨ran.

Characterization and expression of two cDNAs encoding carbonic anhydrase in Arabidopsis by: Expression of carbonic anhydrase (CA) genes in leaves of Arabidopsis plants exposed to different CO 2 concentrations. Total RNA was extracted from leaves collected from plants exposed to (low), (ambient) or (high) ppm CO 2.

Transcripts of AtαCA1‐3 and AtαCA1‐6 were analysed by RT‐PCR. As control, PCR reaction was performed Cited by: 2. CA Gene Expression in Arabidopsis Leaves Gene expression of all family CAs has been demonstrated in Arabidopsis thaliana leaves [10,12], as well as the expression of three family genes encoding -CA1, -CA2 and -CA3 [10].

Rudenko et al. [13] did not detect expression of the latter, but demonstrated that arabidopsis leavesCited by: 1. Carbonic anhydrase (CA; EC [][1]) catalyzes the interconversion of CO2 and HCO3− and is a major protein constituent of the C3 higher plant chloroplast where it is presumed to play a role in photosynthetic carbon by: We demonstrate that Arabidopsis thaliana double-mutant plants in the β-carbonic anhydrases βCA1 and βCA4 show impaired CO 2-regulation of stomatal movements and increased stomatal density, but retain functional abscisic-acid and blue-light responses.

βCA-mediated CO 2-triggered stomatal movements are not, in first-order, linked to whole leaf. Carbonic anhydrase and plant resistance to biotic stresses. There is evidence that in the immune response of plants to pathogens, carbonic anhydrase plays a very important role, acting as a salicylic acid (SA) binding protein 3 – SABP3 (Slaymaker et al., ).Experimentally, this dual activity of chloroplastic CAs has been observed in by: 5.

KEY MESSAGE: Red alga, Gracilariopsis chorda, contains seven carbonic anhydrases that can be grouped into α- β- and γ-classes. Carbonic anhydrases (CAHs) are metalloenzymes that catalyze the reversible hydration of CO2.

These enzymes are present in all living organisms and play roles in various cellular processes, including : Md. Abdur Razzak, JunMo Lee, Dong Wook Lee, Jeong Hee Kim, Hwan Su Yoon, Inhwan Hwang. Abstract. The hypothesis that aquaporins and carbonic anhydrase (CA) are involved in the regulation of stomatal (g s) and mesophyll (g m) conductance to CO 2 was tested in a short-term water-stress and recovery experiment in 5-year-old olive plants (Olea europaea) growing evolution of leaf gas exchange, chlorophyll fluorescence, and plant water status, and a quantitative Cited by:   Other potential roles for the A.

woodii carbonic anhydrase include the regulation of intracellular pH due to the large amount of acetate being produced during growth and the coupling to an acetate/HCO 3 − antiporter. 5 Properties of isolated carbonic anhydrases from the Archaea domainCited by: Two distinct cDNA clones encoding carbonic anhydrase (CA) were isolated from an Arabidopsis thaliana (lambda)YES library.

One of these clones, CA1, encodes a kD polypeptide and is essentially the same as a previously reported Arabidopsis CA cDNA (C.A.

Raines, P.R. Horsnell, C. Holder, J.C. Lloyd () Plant Mol Biol –). Comparison of the derived amino acid .